Development of Reverse Transcriptase PCR and Nested PCR to Detect Porcine Hemagglutinating Encephalomyelitis Virus
نویسندگان
چکیده
منابع مشابه
Nested Reverse Transcriptase-PCR (NRT-PCR) Assay for detection of Classical Swine Fever Virus
Classical Swine Fever(CSF) or Hog Cholera is one of the most feared and devastating disease of pigs. The disease has become main threat to pig industry in counrties with a dense pig population and is known to cause more deaths in pigs as compared to many other infectious agents. The disease is endemic in many parts of India including Assam. The virus belongs to genus Pestivirus and family Flavi...
متن کاملDevelopment of real-time reverse transcriptase PCR assays to detect and serotype dengue viruses.
Serotyping dengue virus (DENV) from suspect human specimens is crucial for developing sound epidemiological control measurements early in the transmission season and for effective patient management. We modified DENV consensus D1 (mD1) and serotype-specific TS2 (mTS2) and redesigned serotype-specific TS1 (rTS1) and TS4 (rTS4) as described previously in the conventional capsid and premembrane ge...
متن کاملDevelopment of a sensitive real-time reverse transcriptase PCR assay with an internal control to detect and quantify chikungunya virus.
BACKGROUND The chikungunya virus (CHIKV; Alphavirus, Togaviridae) has emerged in the south Western Indian Ocean since early 2005. A major outbreak of CHIKV infection occurred in Réunion Island, where the virus is transmitted by Aedes albopictus mosquitoes. Facing an outbreak of unprecedented magnitude, we developed a rapid, sensitive, and reliable assay for the detection and quantification of C...
متن کاملPCR - Based Reverse Transcriptase Assay
ES cells. This phenomenon has been observed in our laboratory in a number of cases. Clones putatively identified as homologous recombinants by Southern analysis can be quickly checked for random insertion of extra copies of the targeting vector by PCR. Given the small difference in size between the two alleles and use of only two primers common to both WT and mutant DNA, the amplification rate ...
متن کاملReverse transcriptase-PCR assay for detection of hog cholera virus.
A reverse transcriptase-PCR strategy was developed for the detection of hog cholera virus. Hog cholera virus template was amplified from tissue culture fluids and from tissues and blood of infected pigs, but not from samples containing other pestiviruses. Restriction endonuclease analysis identified samples as historic or recent isolates.
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2004
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.66.367